Strep-Tactin®XT BLI Coupling Kit
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Strep-Tactin®XT BLI Coupling Kit
Coating of BLI biosensors with Strep-Tactin®XT for reversible capture and analysis of Twin-Strep-tag® proteins.
Strep-Tactin®XT BLI Coupling Kit is intended for site-directed, reversible capture of Twin-Strep-tag® fusion proteins for biomolecular interaction analysis using Bio-Layer Interferometry (BLI). It provides Strep-Tactin®XT, Immobilization Buffer, Ethanolamine, EDC, and s-NHS sufficient for coating of 96 biosensors (1 tray). The pM affinity of Strep-Tactin®XT for Twin-Strep-tag® fusion proteins allows specific capture even from complex samples, e.g., culture supernatants. Coated biosensors can be easily regenerated by application of the provided Regeneration Buffer without loss of the Strep-Tactin®XT coating which enables multiple re-uses.
| Detector/Ligand: | Strep-Tactin®XT |
|---|---|
| Kit Content: | Immobilization Buffer, Regeneration Buffer, 10x Buffer W, Strep-Tactin®XT, Ethanolamine, EDC, s-NHS |
| Possible Application: | Site-directed, reversible capture of Twin-Strep-tag® fusion proteins |
| Specificity: | Twin-Strep-tag®; cross reactivity: biotin |
| Storage: | 2-8 °C, -20 °C |
|---|---|
| Stability: | 6 months after shipping |
| Shipping: | Blue ice |
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