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Strep-Tactin®XT 4Flow® resin
Strep-Tactin®XT 4Flow® 50% suspension is coupled to the high affinity ligand Strep-Tactin®XT and can be used for the purification of Strep-tag®II and Twin-Strep-tag® proteins. The resin offers outstanding target purity (>95%), a high binding affinity and a low agarose concentration. It is recommended for use with all protein classes, especially low abundant or challenging proteins. For proteins with an increased affinity, like those with multiple tags or multimers, we recommend using a Strep-Tactin® resin. The suspension format allows the packing of custom gravity flow columns. For a 1 ml resin bed volume, use 2 ml 50 % suspension.
A great pressure stability enables the use of Strep-Tactin®XT 4Flow® in FPLC setups.
Please review the details of our other resins if you have other needs for your purification.
Specifications
| Bead Size: | 50 - 150 µm spherical |
|---|---|
| Form: | 50% suspension |
| Possible Application: | Protein purification of Strep-tag®II and Twin-Strep-tag® fusion proteins |
| Protein Binding Capacity: | 11 mg/ml resin |
| Specificity: | Twin-Strep-tag® and Strep-tag®II |
| Support: | 4% agarose, crosslinked |
Shipping information
| Storage: | 2-8 °C |
|---|---|
| Stability: | 12 months after shipping |
| Shipping: | Room temperature |
Application example with Strep-Tactin® XT 4Flow®
Packing of a Gravity Flow Column for Protein Purification
Protein Purification Tutorial with Strep-Tactin®XT 4Flow®
Strep-Tactin®XT 4Flow® Color based functionality check
Read more about the Strep-Tactin®XT purification cycle
Read more about Strep-Tactin®XT resin specifications
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