Strep-Tactin® TACS Agarose
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Strep-Tactin® TACS Agarose
50% suspension for isolation of cells and exosomes via affinity chromatography
Strep-Tactin® TACS Agarose is suitable for cell selection or depletion via affinity chromatography. The resin consists of agarose macrobeads coated with the streptavidin variant Strep-Tactin®. A special crosslinking permits the optimal use for cell-related approaches.
Strep-Tactin® TACS Agarose can be loaded with a protein of choice that is fused to a Twin-Strep-tag®. This can be for example an antibody or an antigen, which captures the cells inside the columns. Biotin addition causes the dissociation of strep-tagged proteins from Strep-Tactin®, leading to the elution of cells.
Alternatively, it is possible to load the resin with Twin-Strep-tagged, desthiobiotinylated or biotinylated proteins (irreversible binding) for negative cell selection or depletion.
The suspension can be used for packing of gravity flow columns since the resin is optimized for column affinity chromatography. To pack a column with 1 ml resin bed volume, 2 ml 50% suspension are necessary.
| Biotin Binding Capacity: | > 40 nmol/ml resin |
|---|---|
| Form: | 50% suspension |
| Possible Application: | Cell isolation or depletion via affinity chromatography |
| Support: | Crosslinked agarose |
| Storage: | 2-8 °C |
|---|---|
| Stability: | 6 months after shipping |
| Shipping: | Room temperature |
Accessories
Biotin stock solution
100 mM concentrated biotin for dissociation of Twin-Strep-tag® from Strep-Tactin® suitable for cells
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Strep-Tactin® TACS Agarose Column
Gravity flow column suitable for cell and exosome isolation via affinity chromatography
