IBA’s latest agarose resin, Strep-Tactin® 4Flow® High Capacity
Strep-Tactin® 4Flow® high capacity is our latest agarose resin in our 4Flow® resin product line. This product unifies and replaces the following resins, delivering the same exceptional performance:
- Strep-Tactin® Sepharose® (2-1201-002, 2-1201-010, 2-1201-025, 2-1201-500, 2-1202-550, 2-1202-001)
- Strep-Tactin® Superflow® (2-1206-025)
- Strep-Tactin® Superflow® high capacity (2-1208-002, 2-1208-010, 2-1208-025, 2-1208-500, 2-1209-001, 2-1209-051, 2-1209-101, 2-1209-550, 2-1239-001, 2-1240-001)
Excellent purity and enhanced binding capacity with Strep-Tactin® 4Flow® high capacity
This resin is designed with a low-concentrated (4%) and pressure-stable agarose matrix, coupled with a high Strep-Tactin® density. These unique specifications provide several key advantages:
- High protein binding capacity
- Applicability for FPLC workstations
- High yields for all protein classes, especially large proteins
Strep-Tactin® 4Flow® high capacity unifies features of previous resins
| Matrix | Strep-Tactin® Superflow® | Strep-Tactin® Sepharose® | Strep-Tactin® 4Flow® |
|
Bead structure |
6% agarose, crosslinked | 4% agarose, crosslinked |
4% agarose, highly crosslinked |
|
Bead size |
60-160 μm, spherical | 45-165 μm |
50-150 μm, spherical |
|
Exclusion limit |
6 x 106 Da | ~3 x 107 Da |
3 x 107 Da |
|
Recommended flow
rate | 1 ml FPLC column: 0,5-1 ml/min 5 ml FPLC column: 1-3 ml/min | gravity flow |
1 ml FPLC column: 0,5-1 ml/min 5 ml FPLC column: 1-3 ml/min |
| pH range for protein binding | 7-8 | 7-8 | 7-8 |
| Max pressure | 9.6 bar | gravity flow | 3.5 bar |
| Eluent | desthiobiotin | desthiobiotin | desthiobiotin |
| Regeneration buffer | Buffer R | Buffer R | 100 mM NaOH |
Strep-Tactin® and Strep-Tactin®XT
Strep-Tactin® and Strep-Tactin®XT resins offer exceptional protein purity (>95%) through highly specific binding properties, while preserving the activity of the target protein. Discover more about the high affinity variant, Strep-Tactin®XT, here.
4Flow® resin products
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