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2-1253-001

Strep-Tactin® 4Flow® high capacity column

Replaces Strep-Tactin® Sepharose®, Strep-Tactin® Superflow® and Strep-Tactin® Superflow® high capacity gravity flow columns
Content
Strep-Tactin® 4Flow® high capacity columns replace Strep-Tactin® Sepharose®, Strep-Tactin® Superflow® and Strep-Tactin® Superflow® high capacity columns, providing identical performance and unifying the resins. Further information about the differences between Sepharose®, Superflow®, and 4Flow®, as well as the advantages of Strep-Tactin® 4Flow® high capacity, can be found here.

Strep-Tactin® 4Flow® high capacity columns are designed for purifying Strep-tag®II and Twin-Strep-tag® proteins via affinity chromatography in gravity flow. This product features a 4% cross-linked agarose beads coupled with Strep-Tactin®, a variant of streptavidin. Strep-Tactin® selectively binds to proteins fused with a Strep-tag®II or Twin-Strep-tag® and enables targeted elution via desthiobiotin. It can be regenerated with 100 mM NaOH for multiple uses, offering a cost-effective solution. This product serves as an alternative to our Strep-Tactin®XT 4Flow® resins for proteins that are challenging to elute. Please note that biotinylated proteins also bind to Strep-Tactin® 4Flow® high capacity. For samples containing biotinylated proteins, consider using BioLock. For comprehensive details on our available resins, including recommendations, refer to our resin specifications.

Specifications

Bead Size: 50 - 150 µm spherical
Form: Pre-packed gravity flow column
Possible Application: Protein purification of Strep-tag®II and Twin-Strep-tag® fusion proteins
Protein Binding Capacity: 20 mg/ml resin
Specificity: Twin-Strep-tag®; Strep-tag®II; cross reactivity: biotin
Support: 4% agarose, crosslinked

Shipping information

Storage: 2-8 °C
Stability: 6 months after shipping
Shipping: Room temperature

Accessories

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WET FRED; protein purification
WET FRED Set
Application aid for large volumes of lysates or culture supernatants
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Buffer E (10x)
Buffer E (10x)
Strep-Tactin® elution buffer with desthiobiotin
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Buffer W (10x)
Buffer W (10x)
Strep-Tactin® and Strep-Tactin®XT wash buffer
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Desthiobiotin
Desthiobiotin
Desthiobiotin powder for preparation of Strep-Tactin® elution buffer
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Related Products

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MagStrep® Strep-Tactin® beads
MagStrep® Strep-Tactin® beads
Magnetic beads (5% suspension) to purify BioID targets
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Strep-Tactin® 4Flow® high capacity resin
Strep-Tactin® 4Flow® high capacity resin
Replaces Strep-Tactin® Sepharose®, Strep-Tactin® Superflow® and Strep-Tactin® Superflow® high capacity
Read more
Strep-Tactin® 4Flow® high capacity FPLC column
Strep-Tactin® 4Flow® high capacity FPLC column
Replaces Strep-Tactin® Superflow® high capacity FPLC column
Read more
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