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Strep-Tactin® 4Flow® high capacity column

Strep-Tactin® 4Flow® high capacity columns are designed for purifying Strep-tag®II and Twin-Strep-tag® proteins via affinity chromatography in gravity flow. This product features a 4% cross-linked agarose beads coupled with Strep-Tactin®, a variant of streptavidin. Strep-Tactin® selectively binds to proteins fused with a Strep-tag®II or Twin-Strep-tag® and enables targeted elution via desthiobiotin. It can be regenerated with 100 mM NaOH for multiple uses, offering a cost-effective solution. This product serves as an alternative to our Strep-Tactin®XT 4Flow® resins for proteins that are challenging to elute. Please note that biotinylated proteins also bind to Strep-Tactin® 4Flow® high capacity. For samples containing biotinylated proteins, consider using BioLock. For comprehensive details on our available resins, including recommendations, refer to our resin specifications.

Specifications

Bead Size: 50 - 150 µm spherical
Form: Pre-packed gravity flow column
Possible Application: Protein purification of Strep-tag®II and Twin-Strep-tag® fusion proteins
Protein Binding Capacity: 20 mg/ml resin
Specificity: Twin-Strep-tag®; Strep-tag®II; cross reactivity: biotin
Support: 4% agarose, crosslinked

Shipping information

Storage: 2-8 °C
Stability: 6 months after shipping
Shipping: Room temperature

Accessories

WET FRED Set
Application aid for large volumes of lysates or culture supernatants
Buffer E (10x)
Strep-Tactin® elution buffer with desthiobiotin
Buffer W (10x)
Strep-Tactin® and Strep-Tactin®XT wash buffer
Desthiobiotin
Desthiobiotin powder for preparation of Strep-Tactin® elution buffer

Related Products

MagStrep® Strep-Tactin® beads
Magnetic beads (5% suspension) to purify BioID targets
Strep-Tactin® 4Flow® high capacity resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® 4Flow® high capacity FPLC column
FPLC column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins