Strep-Tactin® 4Flow® high capacity column
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Strep-Tactin® 4Flow® high capacity column
Gravity flow column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® 4Flow® high capacity columns are designed for purifying Strep-tag®II and Twin-Strep-tag® proteins via affinity chromatography in gravity flow. This product features a 4% cross-linked agarose beads coupled with Strep-Tactin®, a variant of streptavidin. Strep-Tactin® selectively binds to proteins fused with a Strep-tag®II or Twin-Strep-tag® and enables targeted elution via desthiobiotin. It can be regenerated with 100 mM NaOH for multiple uses, offering a cost-effective solution. This product serves as an alternative to our Strep-Tactin®XT 4Flow® resins for proteins that are challenging to elute.
Please note that biotinylated proteins also bind to Strep-Tactin® 4Flow® high capacity. For samples containing biotinylated proteins, consider using BioLock.
For comprehensive details on our available resins, including recommendations, refer to our resin specifications.
| Bead Size: | 50 - 150 µm spherical |
|---|---|
| Form: | Pre-packed gravity flow column |
| Possible Application: | Protein purification of Strep-tag®II and Twin-Strep-tag® fusion proteins |
| Protein Binding Capacity: | 20 mg/ml resin |
| Specificity: | Twin-Strep-tag®; Strep-tag®II; cross reactivity: biotin |
| Support: | 4% agarose, crosslinked |
| Storage: | 2-8 °C |
|---|---|
| Stability: | 6 months after shipping |
| Shipping: | Room temperature |
Accessories
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