pCSG-IBA143 vector
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5-5143-001
pCSG-IBA143 vector
Mammalian expression vector encoding an N-terminal His-tag and C-terminal Twin-Strep-tag®
pCSG-IBA143 is a large expression vector with universal features for transient expression of target proteins with an N-terminal His-tag and C-terminal Twin-Strep-tag® as well as for generation of stable mammalian cell lines.
The vector carries an ampicillin resistance cassette for selection of transformed E. coli cells and ColE1 origin for a high plasmid copy number. Extrachromosomal replication in mammalian cells could occur either by origin of replication from Epstein-Barr Virus (oriP) or by SV40 ori. For the former the vector provides the EBNA-1 gene and for the latter the cell line has to be latently infected with SV40 or express the SV40 large T antigen (e.g. HEK293T, COS-1, COS-7). Stable cell lines can be selected by the neomycin resistance gene (NeoR). In addition, the human cytomegalovirus (CMV) immediate-early promoter enables a high-level expression in a wide range of mammalian cells.
Please note that cloning into pCSG-IBA acceptor vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. Besides to the direct cloning of the gene of interest into pCSG-IBA vectors with Esp3I, another option via a so-called entry vector is possible.
Please note that cloning into pCSG-IBA acceptor vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. Besides to the direct cloning of the gene of interest into pCSG-IBA vectors with Esp3I, another option via a so-called entry vector is possible.
| Affinity Tag C'-terminal: | Twin-Strep-tag® |
|---|---|
| Affinity Tag N'-terminal: | 6xHis |
| Cloning Method: | Direct cloning using restriction enzyme Esp3I |
| Concentration: | 250 ng/µl |
| Expression Host: | Mammalian cells |
| Form: | Suspension in TE buffer |
| Possible Application: | Vector for recombinant expression in mammalian cells |
| Promoter: | CMV Promoter |
| Resistance: | Ampicillin, Neomycin |
| Sequence: | See Documents |
| Size: | 10986 bp |
| Storage: | -20 °C |
|---|---|
| Stability: | 12 months after shipping |
| Shipping: | Room temperature |
Accessoires
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