pASG-IBAwt1 vector
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5-4000-001
pASG-IBAwt1 vector
Cytoplasmic E. coli expression vector without affinity tag
The pASG-IBAwt1 vector is designed for expression of target proteins without affinity tag in E. coli.
The vector carries an ampicillin resistance cassette for selection of transformed E. coli cells, F1 and ColE1 origin for a high plasmid copy number, and the inducible tetracycline promoter/operator for the regulated expression of proteins. The vector can be combined with any E. coli strain since the tet-promoter works independently of the genetic background of E. coli. Expression of the recombinant protein is induced by addition of anhydrotetracycline, which is a derivative of tetracycline that does not exhibit antibiotic activity.
Please note that cloning into pASG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pASG-IBA vectors with Esp3I, another option via a so-called Entry Vector is possible.
| Cloning Method: | Direct cloning using restriction enzyme Esp3I |
|---|---|
| Concentration: | 250 ng/µl |
| Expression Host: | E. coli |
| Form: | Suspension in TE buffer |
| Possible Application: | Vector for recombinant expression in E. coli |
| Promoter: | tet Promoter |
| Resistance: | Ampicillin |
| Sequence: | See Documents |
| Size: | 3850 bp |
| Storage: | -20 °C |
|---|---|
| Stability: | 12 months after shipping |
| Shipping: | Room temperature |
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