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Empty Spin Columns

Empty Spin Columns are ideal for rapid protein purification from small sample volumes. They are provided with inserted frits (mesh size 10 µm), screw caps, and snap-off plugs and compatible with conventional 1.5 ml or 2.0 ml reaction tubes. The frit in the lower part of the column ensure that the resin remain in the spin column while sample or buffer flow through rapidly during the centrifugation process. The maximum filling volume is 700 µl. The empty spin columns are offered without resin and purification buffer to facilitate a customer-specific application and experimental design, e.g., if specific buffer conditions are required. For purification of Strep-tag®II or Twin-Strep-tag® fusion proteins, the combination with Strep-Tactin®XT 4Flow® high capacity resin is recommended. 

Specifications

Possible Application: Protein purification of Strep-tag®II and Twin-Strep-tag® fusion proteins
Support: Compatible with all standard 1.5 ml or 2.0 ml tubes

Accessories

Strep-Tactin®XT 4Flow® high capacity resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Buffer W (10x)
Strep-Tactin® and Strep-Tactin®XT wash buffer
Buffer BXT (10x)
Strep-Tactin®XT elution buffer
Biotin
Biotin powder for preparation of Strep-Tactin®XT elution buffer

Related Products

MagStrep® Strep-Tactin®XT beads
Magnetic beads (5% suspension) to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® high capacity column
Gravity flow column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® column
Gravity flow column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins