Strep-Tactin®XT 4Flow® high capacity column
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Strep-Tactin®XT 4Flow® high capacity column
Strep-Tactin®XT 4Flow® high capacity columns are applicable to the purification of Strep-tag®II or Twin-Strep-tag® fusion proteins via gravity flow. It is recommended for proteins of all classes, especially low abundant, large, and highly expressed proteins, due to its pH compatibility, target purity (> 95%), and high binding affinity. Multimeric protein complexes might bind too strongly to our Strep-Tactin®XT 4Flow® high capacity resins, preventing efficient elution. Strep-Tactin® resins are recommended for these proteins.
With Strep-Tactin®XT 4Flow® high capacity, denaturing agents such as urea can be used. In comparison to Strep-Tactin®XT 4Flow®, Strep-Tactin®XT 4Flow® high capacity has a higher density of immobilized Strep-Tactin®XT, which leads to a higher yield of purified protein.
Purify large samples faster with our WET FRED.
Check out our resin specification overview for details about recommended applications.
| Bead Size: | 50 - 150 µm spherical |
|---|---|
| Form: | Pre-packed gravity flow column |
| Possible Application: | Protein purification of Strep-tag®II and Twin-Strep-tag® fusion proteins |
| Protein Binding Capacity: | 31 mg/ml resin |
| Specificity: | Twin-Strep-tag® and Strep-tag®II |
| Support: | 4% agarose, crosslinked |
| Storage: | 2-8 °C |
|---|---|
| Stability: | 6 months after shipping |
| Shipping: | Room temperature |
Application Note
Application example
Video - Protein purification with large sample volumes using WET FRED
Video - Color based functionality check - Strep-Tactin® and Strep-Tactin®XT columns compared
Read more about the Strep-Tactin®XT purification cycle
Read more about Strep-Tactin®XT resin specifications
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