Biotin
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Biotin
Biotin powder for preparation of Strep-Tactin®XT elution buffer
Biotin, also known as vitamin B7 or vitamin H, binds to the binding pockets of streptavidin, Strep-Tactin® and Strep-Tactin®XT with different affinites. It can be used for preparation of Strep-Tactin®XT elution buffer, Buffer BXT. Applied to Strep-Tactin®XT resin, it displaces bound Strep-tag®II or Twin-Strep-tag® proteins, allowing an efficient elution.
It can also be used for eluting biotinylated proteins from Strep-Tactin® resins. Please note Strep-Tactin®resins cannot be regenerated after biotin application.
| Alternative Names: | Biotin (vitamin B7, vitamin H, coenzyme R) |
|---|---|
| Form: | Powder |
| Molecular Weight: | 244.3 g/mol |
| Possible Application: | Competitive elution of Strep-tag®II and Twin-Strep-tag® fusion proteins from Strep-Tactin®XT resins |
| Storage: | 2-8 °C |
|---|---|
| Stability: | 12 months after shipping |
| Shipping: | Room temperature |
Accessories
MagStrep® Strep-Tactin®XT beads
Magnetic beads (5% suspension) to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® high capacity resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® column
Gravity flow column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® high capacity column
Gravity flow column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® FPLC column
FPLC column to purify Strep-Tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® high capacity FPLC column
FPLC column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
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