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pLSG-IBA62 vector

The pLSG-IBA62 vector allows the expression of FLAG/[i]Strep[/i]-tag®-fusion-proteins in insect cells. The vector carries the Polyhedrin promoter for high-level expression in insect cells, the FLAG-tag for for N-terminal and the [i]Strep[/i] -tag® for C-terminal fusion to the recombinant protein. The Co-transfection with BacPAK6 linearized AcMNPV DNA (Clontech) or with circular flashBAC modified AcMNPV DNA (Oxford Expression Technologies) allows the generation of recombinant baculovirus at very high efficiency through reconstitution of an essential gene (ORF 1629) and elimination of wild-type virus to great extent. For selection and propagation in [i]E.coli[/i] the vector carries an Ampicillin resistance and ColE1 origin of replication (pUC). The expressed recombinant protein will be localized in the cytoplasm.

Spezifikationen

Affinitätstag (C-terminal): Strep-tag®II
Affinitätstag (N-terminal): FLAG
Expressionswirt: Insektenzellen
Form: Suspension in TE Puffer
Klonierungsmethode: Direkte Klonierung mit Restriktionsenzym Esp3I
Konzentration: 250 ng/µl
Mögliche Anwendungen: Vector for recombinant expression in insect cells
Plasmidgröße: 6218 bp
Promoter: Polyhedrin Promoter
Resistenz: Ampicillin
Sequenz: Siehe Dokumente

Lieferinformationen

Haltbarkeit: 12 Monate nach Versand
Lagerung: -20 °C
Versand: Raumtemperatur

Accessories

Competent cells E. coli TOP10
Chemisch kompetente Zellen für die Plasmidvermehrung
pENTRY-IBA51 vector
Entry vector for generation of StarGate donor vectors

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