pLSG-IBA123 vector
Powered by Bioz
The pLSG-IBA123 vector allows the expression of Twin-Strep®/GST-tag-fusion-proteins in insect cells. The vector carries the Polyhedrin promoter for high-level expression in insect cells, the Twin-Strep-tag® for C-terminal and the GST-tag for N-terminal fusion to the recombinant protein. The Co-transfection with BacPAK6 linearized AcMNPV DNA (Clontech) or with circular flashBAC modified AcMNPV DNA (Oxford Expression Technologies) allows the generation of recombinant baculovirus at very high efficiency through reconstitution of an essential gene (ORF 1629) and elimination of wild-type virus to great extent. For selection and propagation in [i]E.coli[/i] the vector carries an Ampicillin resistance and ColE1 origin of replication (pUC). The expressed recombinant protein will be localized in the cytoplasm. The additional PreScission protease cleavage site allows the removal of the GST-tag.
| Affinitätstag (C-terminal): | Twin-Strep-tag® |
|---|---|
| Affinitätstag (N-terminal): | GST (removal via PreScission® Protease site) |
| Expressionswirt: | Insektenzellen |
| Form: | Suspension in TE Puffer |
| Klonierungsmethode: | Direkte Klonierung mit Restriktionsenzym Esp3I |
| Konzentration: | 250 ng/µl |
| Mögliche Anwendungen: | Vector for recombinant expression in insect cells |
| Plasmidgröße: | 6941 bp |
| Promoter: | Polyhedrin Promoter |
| Resistenz: | Ampicillin |
| Sequenz: | Siehe Dokumente |
| Haltbarkeit: | 12 Monate nach Versand |
|---|---|
| Lagerung: | -20 °C |
| Versand: | Raumtemperatur |
Accessories
Related Products
pLSG-IBA23 vector
Insect expression vector encoding an N-terminal GST-tag and C-terminal Strep-tag®II
