pESG-IBA45 vector
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The pESG-IBA45 vector allows the expression of [i]Strep[/i]-/6x[i]Histidine[/i]-tag-fusion-proteins in Mammalia. The vector carries the Human cytomegalovirus (CMV) immediate-early promoter for high-level expression of proteins, the [i]Strep[/i]-tag® for N-terminal and the 6xHistidine-tag for C-terminal fusion to the recombinant protein as well as the Neomycin resistance gene for selection of stable cell lines. The expressed recombinant protein will be localized in the cytoplasm.
| Affinitätstag (C-terminal): | 6xHis |
|---|---|
| Affinitätstag (N-terminal): | Strep-tag®II |
| Expressionswirt: | Mammaliazellen |
| Form: | Suspension in TE Puffer |
| Klonierungsmethode: | Direkte Klonierung mit Restriktionsenzym Esp3I |
| Konzentration: | 250 ng/µl |
| Mögliche Anwendungen: | Vektor für rekombinante Expression in Mammaliazellen |
| Palsmidgröße: | 6124 bp |
| Promoter: | CMV Promoter |
| Resistenz: | Ampicillin, Neomycin |
| Sequenz: | Siehe Dokumente |
| Haltbarkeit: | 12 Monate nach Versand |
|---|---|
| Lagerung: | -20 °C |
| Versand: | Raumtemperatur |
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