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pESG-IBA162 vector

The pESG-IBA162 vector allows the expression of FLAG/Twin-Strep-tag®-fusion-proteins in Mammalia. The vector carries the Human cytomegalovirus (CMV) immediate-early promoter for high-level expression of proteins, the FLAG-tag for N-terminal and the Twin-Strep-tag® for C-terminal fusion to the recombinant protein as well as the Neomycin resistance gene for selection of stable cell lines. The expressed recombinant protein will be localized in the cytoplasm.

Spezifikationen

Affinitätstag (C-terminal): Twin-Strep-tag®
Affinitätstag (N-terminal): FLAG
Expressionswirt: Mammaliazellen
Form: Suspension in TE Puffer
Klonierungsmethode: Direkte Klonierung mit Restriktionsenzym Esp3I
Konzentration: 250 ng/µl
Mögliche Anwendungen: Vektor für rekombinante Expression in Mammaliazellen
Palsmidgröße: 6187 bp
Promoter: CMV Promoter
Resistenz: Ampicillin, Neomycin
Sequenz: Siehe Dokumente

Lieferinformationen

Haltbarkeit: 12 Monate nach Versand
Lagerung: -20 °C
Versand: Raumtemperatur

Accessories

Competent cells E. coli TOP10
Chemisch kompetente Zellen für die Plasmidvermehrung
pENTRY-IBA51 vector
Entry vector for generation of StarGate donor vectors

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