pESG-IBA144 vector
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The pESG-IBA144 vector allows the expression of Twin-Strep®/6x[i]Histidine[/i] -tag-fusion-proteins in Mammalia. The vector carries the Human cytomegalovirus (CMV) immediate-early promoter for high-level expression of proteins, BM40 signal for secretion of the recombinant protein, the Twin-Strep-tag® for N-terminal and the 6x[i]Histidine[/i]-tag for C-terminal fusion to the recombinant protein as well as the Neomycin resistance gene for selection of stable cell lines. The expressed recombinant protein will be secreted into the medium.
| Affinitätstag (C-terminal): | 6xHis |
|---|---|
| Affinitätstag (N-terminal): | Twin-Strep-tag® |
| Expressionswirt: | Mammaliazellen |
| Form: | Suspension in TE Puffer |
| Klonierungsmethode: | Direkte Klonierung mit Restriktionsenzym Esp3I |
| Konzentration: | 250 ng/µl |
| Mögliche Anwendungen: | Vektor für rekombinante Expression in Mammaliazellen |
| Palsmidgröße: | 6238 bp |
| Promoter: | CMV Promoter |
| Resistenz: | Ampicillin, Neomycin |
| Sequenz: | Siehe Dokumente |
| Haltbarkeit: | 12 Monate nach Versand |
|---|---|
| Lagerung: | -20 °C |
| Versand: | Raumtemperatur |
Accessories
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pCSG-IBA144 vector
Mammalian expression vector for secretion of N-terminal Twin-Strep-tag® and C-terminal His-tag fusion proteins
