pESG-IBA143 vector
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The pESG-IBA143 vector allows the expression of 6x[i]Histidine[/i]/Twin-Strep-tag®-fusion-proteins in Mammalia. The vector carries the Human cytomegalovirus (CMV) immediate-early promoter for high-level expression of proteins, the 6x[i]Histidine[/i] -tag for N-terminal and the Twin-Strep-tag® for C-terminal fusion to the recombinant protein as well as the Neomycin resistance gene for selection of stable cell lines.
The expressed recombinant protein will be localized in the cytoplasm.
The expressed recombinant protein will be localized in the cytoplasm.
| Affinitätstag (C-terminal): | Twin-Strep-tag® |
|---|---|
| Affinitätstag (N-terminal): | 6xHis |
| Expressionswirt: | Mammaliazellen |
| Form: | Suspension in TE Puffer |
| Klonierungsmethode: | Direkte Klonierung mit Restriktionsenzym Esp3I |
| Konzentration: | 250 ng/µl |
| Mögliche Anwendungen: | Vektor für rekombinante Expression in Mammaliazellen |
| Palsmidgröße: | 6181 bp |
| Promoter: | CMV Promoter |
| Resistenz: | Ampicillin, Neomycin |
| Sequenz: | Siehe Dokumente |
| Haltbarkeit: | 12 Monate nach Versand |
|---|---|
| Lagerung: | -20 °C |
| Versand: | Raumtemperatur |
Accessories
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pCSG-IBA145 vector
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