pESG-IBA123 vector
Powered by Bioz
The pESG-IBA123 vector allows the expression of GST/Twin-Strep-tag®-fusion-proteins in Mammalia. The vector carries the Human cytomegalovirus (CMV) immediate-early promoter for high-level expression of proteins, the GST-tag for N-terminal and the Twin-Strep-tag® for C-terminal fusion to the recombinant protein as well as the Neomycin resistance gene for selection of stable cell lines. The expressed recombinant protein will be localized in the cytoplasm. The additional PreScission protease cleavage site allows the removal of the GST-tag.
| Affinitätstag (C-terminal): | Twin-Strep-tag® |
|---|---|
| Affinitätstag (N-terminal): | GST (removal via PreScission® Protease site) |
| Expressionswirt: | Mammaliazellen |
| Form: | Suspension in TE Puffer |
| Klonierungsmethode: | Direkte Klonierung mit Restriktionsenzym Esp3I |
| Konzentration: | 250 ng/µl |
| Mögliche Anwendungen: | Vektor für rekombinante Expression in Mammaliazellen |
| Palsmidgröße: | 6850 bp |
| Promoter: | CMV Promoter |
| Resistenz: | Ampicillin, Neomycin |
| Sequenz: | Siehe Dokumente |
| Haltbarkeit: | 12 Monate nach Versand |
|---|---|
| Lagerung: | -20 °C |
| Versand: | Raumtemperatur |
Accessories
Related Products
pESG-IBA23 vector
Mammalian expression vector encoding an N-terminal GST-tag and C-terminal Strep-tag®II
pCSG-IBA23 vector
Mammalian expression vector encoding an N-terminal GST-tag and C-terminal Strep-tag®II
pCSG-IBA123 vector
Mammalian expression vector encoding an N-terminal GST-tag and C-terminal Twin-Strep-tag®
