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pESG-IBA105 vector

The pESG-IBA105 vector allows the expression of Twin-Strep-tag®-fusion-proteins in Mammalia. The vector carries the Human cytomegalovirus (CMV) immediate-early promoter for high-level expression of proteins, the Twin-Strep-tag® for N-terminal fusion to the recombinant protein as well as the Neomycin resistance gene for selection of stable cell lines. The expressed recombinant protein will be localized in the cytoplasm.

Spezifikationen

Affinitätstag (N-terminal): Twin-Strep-tag®
Expressionswirt: Mammaliazellen
Form: Suspension in TE Puffer
Klonierungsmethode: Direkte Klonierung mit Restriktionsenzym Esp3I
Konzentration: 250 ng/µl
Mögliche Anwendungen: Vektor für rekombinante Expression in Mammaliazellen
Palsmidgröße: 6163 bp
Promoter: CMV Promoter
Resistenz: Ampicillin, Neomycin
Sequenz: Siehe Dokumente

Lieferinformationen

Haltbarkeit: 12 Monate nach Versand
Lagerung: -20 °C
Versand: Raumtemperatur

Accessories

Competent cells E. coli TOP10
Chemisch kompetente Zellen für die Plasmidvermehrung
Entry vector for generation of StarGate donor vectors

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