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The pESG-IBA105 vector allows the expression of Twin-Strep-tag®-fusion-proteins in Mammalia. The vector carries the Human cytomegalovirus (CMV) immediate-early promoter for high-level expression of proteins, the Twin-Strep-tag® for N-terminal fusion to the recombinant protein as well as the Neomycin resistance gene for selection of stable cell lines. The expressed recombinant protein will be localized in the cytoplasm.
Spezifikationen
| Affinitätstag (N-terminal): | Twin-Strep-tag® |
|---|---|
| Expressionswirt: | Mammaliazellen |
| Form: | Suspension in TE Puffer |
| Klonierungsmethode: | Direkte Klonierung mit Restriktionsenzym Esp3I |
| Konzentration: | 250 ng/µl |
| Mögliche Anwendungen: | Vektor für rekombinante Expression in Mammaliazellen |
| Plasmidgröße: | 6163 bp |
| Promoter: | CMV Promoter |
| Resistenz: | Ampicillin, Neomycin |
| Sequenz: | Siehe Dokumente |
Lieferinformationen
| Haltbarkeit: | 12 Monate nach Versand |
|---|---|
| Lagerung: | -20 °C |
| Versand: | Raumtemperatur |
Accessories
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Competent cells E. coli TOP10
Chemisch kompetente Zellen für die Plasmidvermehrung
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