pCSG-IBA64 vector
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The pCSG-IBA64 vector allows the high-level expression of [i]Strep[/i]/FLAG-tag-fusion-proteins in mammalian hosts under G418 (Geneticin) selection. The vector carries the Human cytomegalovirus (CMV) immediate-early promoter for high-level expression of proteins in a wide range of mammalian cells, the [i]Strep[/i]-tag® for N- terminal and the FLAG-tag for C-terminal fusion of the recombinant protein. The episomal replication is mediated through the Epstein Barr Virus replication origin (oriP) and nuclear antigen encoded by EBNA-1 in human, primate and canine cells and through SV40 replication origin in cells latently infected with SV40 or that express the SV40 large T antigen (e.g. COS-1, COS-7). For selection and propagation in [i]E.coli[/i] the vector carries an Ampicillin resistance and ColE1 origin of replication (pUC). The expressed recombinant protein will be localized in the cytoplasm.
| Affinitätstag (C-terminal): | FLAG |
|---|---|
| Affinitätstag (N-terminal): | Strep-tag®II |
| Expressionswirt: | Mammaliazellen |
| Form: | Suspension in TE Puffer |
| Klonierungsmethode: | Direkte Klonierung mit Restriktionsenzym Esp3I |
| Konzentration: | 250 ng/µl |
| Mögliche Anwendungen: | Vektor für rekombinante Expression in Mammaliazellen |
| Plasmidgröße: | 10935 bp |
| Promoter: | CMV Promoter |
| Resistenz: | Ampicillin, Neomycin |
| Sequenz: | Siehe Dokumente |
| Haltbarkeit: | 12 Monate nach Versand |
|---|---|
| Lagerung: | -20 °C |
| Versand: | Raumtemperatur |
Accessories
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pESG-IBA64 vector
Mammalian expression vector encoding an N-terminal Strep-tag®II and C-terminal FLAG-tag
pCSG-IBA62 vector
Mammalian expression vector encoding an N-terminal FLAG-tag and C-terminal Strep-tag®II
