pCSG-IBA123 vector
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The pCSG-IBA123 vector allows the high-level expression of GST/Twin-Strep-tag®-fusion-proteins in mammalian hosts under G418 (Geneticin) selection. The vector carries the Human cytomegalovirus (CMV) immediate-early promoter for high-level expression of proteins in a wide range of mammalian cells, the Twin-Strep-tag® for C-terminal and the GST-tag for N-terminal fusion of the recombinant protein. The episomal replication is mediated through the Epstein Barr Virus replication origin (oriP) and nuclear antigen encoded by EBNA-1 in human, primate and canine cells and through SV40 replication origin in cells latently infected with SV40 or that express the SV40 large T antigen (e.g. COS-1, COS-7). For selection and propagation in [i]E.coli[/i] the vector carries an Ampicillin resistance and ColE1 origin of replication (pUC). The additional PreScission protease cleavage site allows the removal of the GST-tag. The expressed recombinant protein will be localized in the cytoplasm.
| Affinitätstag (C-terminal): | Twin-Strep-tag® |
|---|---|
| Affinitätstag (N-terminal): | GST (removal via PreScission® Protease site) |
| Expressionswirt: | Mammaliazellen |
| Form: | Suspension in TE Puffer |
| Klonierungsmethode: | Direkte Klonierung mit Restriktionsenzym Esp3I |
| Konzentration: | 250 ng/µl |
| Mögliche Anwendungen: | Vektor für rekombinante Expression in Mammaliazellen |
| Palsmidgröße: | 11576 bp |
| Promoter: | CMV Promoter |
| Resistenz: | Ampicillin, Neomycin |
| Sequenz: | Siehe Dokumente |
| Haltbarkeit: | 12 Monate nach Versand |
|---|---|
| Lagerung: | -20 °C |
| Versand: | Raumtemperatur |
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