pASK-IBA6C vector
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The pASK-IBA6C vector allows the expression of [i]Strep[/i]-tag®-fusion-proteins in [i]E.coli[/i].
The vector carries the inducible tetracycline promoter/operator for the regulated expression of proteins, the ompA signal for periplasmic secretion of the recombinant protein, the [i]Strep[/i]-tag® for N-terminal fusion to the recombinant protein and the Chloramphenicol Resistance cassette. It can be used with any [i]E. coli[/i] strain because the [i]tet[/i]-promoter works independently from the genetic background of [i]E.coli[/i]. The additional factor Xa cleavage site allows the removal of the [i]Strep[/i]-tag®.
- [b]promoter[/b] from bp 37 to 72
- [b]forward primer binding site[/b] from bp 57 to 76
- [b]OmpA signal sequence[/b] from bp 139 to 201
- [b][i]Strep[/i]-tag®[/b] from bp 202 to 231
- [b]factor Xa cleavage site[/b] from bp 232 to 243
- [b]multiple cloning site[/b] from bp 244 to 320
- [b]reverse primer binding site[/b] from bp 388 to 404
- [b]f1 origin[/b] from bp 417 to 855
- [b]CamR resistance gene[/b] from bp 977 to 1636
- [b][i]tet[/i]-repressor[/b] from bp 1649 to 2272
The vector carries the inducible tetracycline promoter/operator for the regulated expression of proteins, the ompA signal for periplasmic secretion of the recombinant protein, the [i]Strep[/i]-tag® for N-terminal fusion to the recombinant protein and the Chloramphenicol Resistance cassette. It can be used with any [i]E. coli[/i] strain because the [i]tet[/i]-promoter works independently from the genetic background of [i]E.coli[/i]. The additional factor Xa cleavage site allows the removal of the [i]Strep[/i]-tag®.
- [b]promoter[/b] from bp 37 to 72
- [b]forward primer binding site[/b] from bp 57 to 76
- [b]OmpA signal sequence[/b] from bp 139 to 201
- [b][i]Strep[/i]-tag®[/b] from bp 202 to 231
- [b]factor Xa cleavage site[/b] from bp 232 to 243
- [b]multiple cloning site[/b] from bp 244 to 320
- [b]reverse primer binding site[/b] from bp 388 to 404
- [b]f1 origin[/b] from bp 417 to 855
- [b]CamR resistance gene[/b] from bp 977 to 1636
- [b][i]tet[/i]-repressor[/b] from bp 1649 to 2272
| Affinitätstag (N-terminal): | Strep-tag®II |
|---|---|
| Expressionswirt: | E. coli |
| Form: | Suspension in TE Puffer |
| Klonierungsmethode: | Direkte Klonierung mit Restriktionsenzym, z.B. BsaI in MCS |
| Konzentration: | 250 ng/µl |
| Mögliche Anwendungen: | Expressionsplasmid für Strep-tag®II Fusionsproteine |
| Palsmidgröße: | 3081 bp |
| Promoter: | tet Promoter |
| Resistenz: | Chloramphenicol |
| Sequenz: | Siehe Dokumente |
| Spezifität: | Factor Xa Schnittstelle |
| Haltbarkeit: | 12 Monate nach Versand |
|---|---|
| Lagerung: | -20 °C |
| Versand: | Raumtemperatur |
Accessories
Anhydrotetracycline
Tetracyclin-Derivat zur Induktion von Tetracyclin-gesteuerten Promotoren
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