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pASK-IBA2C vector

The pASK-IBA2C vector allows the expression of [i]Strep[/i]-tag®-fusion-proteins in [i]E.coli[/i].
The vector carries the inducible tetracycline promoter/operator for the regulated expression of proteins and the ompA signal for periplasmic secretion of the recombinant protein, the [i]Strep[/i]-tag® for C-terminal fusion to the recombinant protein and the Chloramphenicol Resistance cassette. It can be used with any [i]E. coli[/i] strain because the [i]tet[/i]-promoter works independently from the genetic background of [i]E.coli[/i].

- [b]promoter[/b] from bp 37  to 72 
- [b]forward primer binding site[/b] from bp 57  to 76 
- [b]OmpA signal sequence[/b] from bp 139 to 201
- [b]multiple cloning site[/b] from bp 202 to 282
- [b][i]Strep[/i]-tag®[/b] from bp 283 to 312
- [b]reverse primer binding site from bp 407 to 423
- [b]f1 origin[/b] from bp 436 to 874
- [b]CamR resistance gene[/b] from bp 1023 to 1883
- [b][i]tet[/i]-repressor[/b] from bp 1893 to 2516

Spezifikationen

Affinitätstag (C-terminal): Strep-tag®II
Expressionswirt: E. coli
Form: Suspension in TE Puffer
Klonierungsmethode: Direkte Klonierung mit Restriktionsenzym, z.B. BsaI in MCS
Konzentration: 250 ng/µl
Mögliche Anwendungen: Expressionsplasmid für Strep-tag®II Fusionsproteine
Palsmidgröße: 3061 bp
Promoter: tet Promoter
Resistenz: Chloramphenicol
Sequenz: Siehe Dokumente

Lieferinformationen

Haltbarkeit: 12 Monate nach Versand
Lagerung: -20 °C
Versand: Raumtemperatur

Accessories

Anhydrotetracycline
Tetracyclin-Derivat zur Induktion von Tetracyclin-gesteuerten Promotoren
Competent cells E. coli TOP10
Chemisch kompetente Zellen für die Plasmidvermehrung

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