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pASG-IBAwt1 vector

The pASG-IBAwt1 vector allows the expression of fusion-proteins in [i]E.coli[/i]. The vector carries the inducible tetracycline promoter/operator for the regulated expression of proteins and the Ampicillin Resistance cassette. It can be used with any [i]E.coli[/i] strain because the tet-promoter works independently from the genetic background of [i]E.coli[/i].

The expressed recombinant protein will be localized in the cytoplasm.
This vector does not contain an affinity tag.

Spezifikationen

Expressionswirt: E. coli
Form: Suspension in TE Puffer
Klonierungsmethode: Direkte Klonierung mit Restriktionsenzym Esp3I
Konzentration: 250 ng/µl
Mögliche Anwendungen: Vektor for rekombinante Expression in E. coli
Palsmidgröße: 3850 bp
Promoter: tet Promoter
Resistenz: Ampicillin
Sequenz: Siehe Dokumente

Lieferinformationen

Haltbarkeit: 12 Monate nach Versand
Lagerung: -20 °C
Versand: Raumtemperatur

Accessories

Competent cells E. coli TOP10
Chemisch kompetente Zellen für die Plasmidvermehrung
pENTRY-IBA51 vector
Entry vector for generation of StarGate donor vectors

Related Products

pASG-IBAwt2 vector
Periplasmic E. coli expression vector without affinity tag