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The pASG-IBA5 vector allows the expression of [i]Strep[/i]-tag®-fusion-proteins in [i]E.coli[/i]. The vector carries the inducible tetracycline promoter/operator for the regulated expression of proteins, the [i]Strep[/i]-tag® for N-terminal fusion to the recombinant protein and the Ampicillin Resistance cassette. It can be used with any [i]E. coli[/i] strain because the tet-promoter works independently from the genetic background of [i]E.coli[/i].
The expressed recombinant protein will be localized in the cytoplasm.
The protein will be fused with a N-terminal Strep-tagII.
The expressed recombinant protein will be localized in the cytoplasm.
The protein will be fused with a N-terminal Strep-tagII.
Spezifikationen
| Affinitätstag (N-terminal): | Strep-tag®II |
|---|---|
| Expressionswirt: | E. coli |
| Form: | Suspension in TE Puffer |
| Klonierungsmethode: | Direkte Klonierung mit Restriktionsenzym Esp3I |
| Konzentration: | 250 ng/µl |
| Mögliche Anwendungen: | Vektor for rekombinante Expression in E. coli |
| Plasmidgröße: | 3892 bp |
| Promoter: | tet Promoter |
| Resistenz: | Ampicillin |
| Sequenz: | Siehe Dokumente |
Lieferinformationen
| Haltbarkeit: | 12 Monate nach Versand |
|---|---|
| Lagerung: | -20 °C |
| Versand: | Raumtemperatur |
Accessories
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Competent cells E. coli TOP10
Chemisch kompetente Zellen für die Plasmidvermehrung
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Cytoplasmic E. coli expression vector encoding a C-terminal Strep-tag®II
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pASK-IBA3C vector
Cytoplasmic E. coli expression vector encoding a C-terminal Strep-tag®II and chloramphenicol resistance
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pASK-IBA5C
Cytoplasmic E. coli expression vector encoding an N-terminal Strep-tag®II and chloramphenicol resistance
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pASK-IBA7C vector
Cytoplasmic E. coli expression vector encoding an N-terminal Strep-tag®II, chloramphenicol resistance and protease cleavage site
