pASG-IBA4 vector
The pASG-IBA4 vector allows the expression of [i]Strep[/i]-tag®-fusion-proteins in [i]E.coli[/i]. The vector carries the inducible tetracycline promoter/operator for the regulated expression of proteins, the ompA signal for periplasmic secretion of the recombinant protein, the [i]Strep[/i]-tag® for N-terminal fusion to the recombinant protein and the Ampicillin Resistance cassette. It can be used with any [i]E.coli[/i] strain because the tet-promoter works independently from the genetic background of [i]E.coli[/i].
Affinitätstag (N-terminal): | Strep-tag®II |
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Expressionswirt: | E. coli |
Form: | Suspension in TE Puffer |
Klonierungsmethode: | Direkte Klonierung mit Restriktionsenzym Esp3I |
Konzentration: | 250 ng/µl |
Mögliche Anwendungen: | Vektor for rekombinante Expression in E. coli |
Palsmidgröße: | 3937 bp |
Promoter: | tet Promoter |
Resistenz: | Ampicillin |
Sequenz: | Siehe Dokumente |
Haltbarkeit: | 12 Monate nach Versand |
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Lagerung: | -20 °C |
Versand: | Raumtemperatur |