pASG-IBA123 vector
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The pASG-IBA123 vector allows the expression of Twin-Strep®-/GST-tag-fusion-proteins in [i]E.coli[/i]. The vector carries the inducible tetracycline promoter/operator for the regulated expression of proteins, the Twin-Strep-tag® for C-terminal and the GST-tag for N-terminal fusion to the recombinant protein as well as the Ampicillin Resistance cassette. It can be used with any [i]E.coli[/i] strain because the tet-promoter works independently from the genetic background of [i]E.coli[/i]. The additional PreScission protease cleavage site allows the removal of the GST-tag.
The expressed recombinant protein will be localized in the cytoplasm.
The expressed recombinant protein will be localized in the cytoplasm.
| Affinitätstag (C-terminal): | Twin-Strep-tag® |
|---|---|
| Affinitätstag (N-terminal): | GST (removal via PreScission® Protease site) |
| Expressionswirt: | E. coli |
| Form: | Suspension in TE Puffer |
| Klonierungsmethode: | Direkte Klonierung mit Restriktionsenzym Esp3I |
| Konzentration: | 250 ng/µl |
| Mögliche Anwendungen: | Vektor for rekombinante Expression in E. coli |
| Palsmidgröße: | 4639 bp |
| Promoter: | tet Promoter |
| Resistenz: | Ampicillin |
| Sequenz: | Siehe Dokumente |
| Haltbarkeit: | 12 Monate nach Versand |
|---|---|
| Lagerung: | -20 °C |
| Versand: | Raumtemperatur |
Accessories
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