Competent E. coli TOP10
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5-1600-020
Competent E. coli TOP10
Chemically competent cells for plasmid propagation
Competent E. coli TOP10 cells are ready for heat shock transformation with vector DNA and its subsequent propagation for cloning and transfection purposes. The chemically competent cells are provided as 20 separate one-shot reactions. Transformed cells can be selected by blue/white screening.
The genotype of Competent E. coli TOP10 cells is F- mcrA (mrr-hsdRMS-mcrBC) 80lacZ M15 lacX74 recA1 ara 139 (ara-leu)7697 galU galK rpsL (StrR) endA1 nupG.
| Biosafety Level: | S1 (Germany), BSL-2 (USA) |
|---|---|
| Form: | Cryopreserved |
| Possible Application: | Expression host for recombinat Strep-tag®II and Twin-Strep-tag® proteins |
| Storage: | -80 °C |
|---|---|
| Stability: | 12 months after shipping |
| Shipping: | Dry ice |
Accessories
pASK-IBA3C vector
Cytoplasmic E. coli expression vector encoding a C-terminal Strep-tag®II and chloramphenicol resistance
pASG-IBA3 vector
Cytoplasmic E. coli expression vector encoding a C-terminal Strep-tag®II
pDSG-IBA103 vector
Mammalian expression vector encoding a C-terminal Twin-Strep-tag®
