Buffer R (10x)
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Buffer R (10x) is a 10x concentrated Strep-Tactin® regeneration buffer containing HABA (2-[4'-hydroxy-benzeneazo]benzoic acid). HABA displaces desthiobiotin from the modified biotin-binding pocket of Strep-Tactin® Sepharose®, Strep-Tactin® Superflow® and Strep-Tactin® Superflow® high capacity after protein elution. Upon binding of HABA to Strep-Tactin®, the yellow buffer turns red, indicating regeneration and column activity. Please note that 10x Buffer R cannot be used for the regeneration of Strep-Tactin® 4Flow® high capacity and Strep-Tactin®XT resins, but it can be used to monitor the resin's activity status after the regeneration.
| Concentration: | 10x concentrated |
|---|---|
| Form: | Solution |
| Possible Application: | Regeneration of Strep-Tactin® Sepharose®, Strep-Tactin® Superflow® and Strep-Tactin® Superflow® high capacity |
| Storage: | 2-8 °C |
|---|---|
| Stability: | 12 months after shipping |
| Shipping: | Room temperature |
Accessories
Strep-Tactin® MacroPrep® resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
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